3WPX

Structure of PomBc4, a periplasmic fragment of PomB from Vibrio alginolyticus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	CCD
Collection date	2011-02-16
Detector	RAYONIX MX225HE
Wavelength(s)	0.97910
Spacegroup name	P 21 21 21
Unit cell lengths	58.408, 63.300, 77.213
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.960 - 2.300
R-factor	0.1812
Rwork	0.175
R-free	0.23410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3wpw
RMSD bond length	0.006
RMSD bond angle	1.010
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.600	2.420
High resolution limit [Å]	2.300	2.300
Rmerge	0.099	0.391
Number of reflections	12786
<I/σ(I)>	9.7	3.7
Completeness [%]	97.2	98.5
Redundancy	4.6	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	20%(w/v) PEGMME2000, 0.1M imidazole, 4%(v/v) MPD, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K