3WP8
Acinetobacter sp. Tol 5 AtaA C-terminal Ylhead fused to GCN4 adaptors (Chead)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 63 |
| Unit cell lengths | 54.406, 54.406, 210.732 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.240 - 1.970 |
| R-factor | 0.1782 |
| Rwork | 0.175 |
| R-free | 0.22980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ntn |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.764 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.070 |
| High resolution limit [Å] | 1.970 | 4.310 | 2.000 |
| Rmerge | 0.069 | 0.038 | 0.421 |
| Number of reflections | 24269 | ||
| <I/σ(I)> | 14.8 | ||
| Completeness [%] | 99.2 | 98 | 99 |
| Redundancy | 11.2 | 11.1 | 11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.6 | 298 | 3% PEG 6000, 1.0M NaCl, 0.1M Na-acetate, pH 3.6, vapor diffusion, hanging drop, temperature 298K |
