3WOF

Crystal structure of P23-45 gp39 (6-132) bound to Thermus thermophilus RNA polymerase beta-flap domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NE3A
Synchrotron site	Photon Factory
Beamline	AR-NE3A
Temperature [K]	100
Detector technology	CCD
Collection date	2012-02-11
Detector	ADSC QUANTUM 270
Spacegroup name	P 41 21 2
Unit cell lengths	213.777, 213.777, 234.598
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.549 - 3.298
R-factor	0.2205
Rwork	0.219
R-free	0.24640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3woe
RMSD bond length	0.009
RMSD bond angle	1.227
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.298
Number of reflections	82014
<I/σ(I)>	14.2
Completeness [%]	99.9
Redundancy	10.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.6	293	50mM MES-NaOH (pH 5.6), 6% PEG 8000, 200mM KCl, 10mM MgCl2, VAPOR DIFFUSION, SITTING DROP, temperature 293K