3WN5
Crystal structure of asymmetrically engineered Fc variant in complex with FcgRIIIa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2012-02-17 |
| Detector | ADSC QUANTUM 270 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.030, 72.490, 163.480 |
| Unit cell angles | 90.00, 91.15, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.780 |
| R-factor | 0.238 |
| Rwork | 0.236 |
| R-free | 0.27400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.947 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.030 | 2.850 |
| High resolution limit [Å] | 2.780 | 2.780 |
| Rmerge | 0.079 | 0.619 |
| Number of reflections | 43675 | |
| <I/σ(I)> | 13.91 | 2.09 |
| Completeness [%] | 98.0 | 98.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.25 | 293 | 14.5% PEG 3350, 0.1M Bis-Tris, 0.2M Sodium Iodide, Streak seeding, pH 6.25, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
