3WN5
Crystal structure of asymmetrically engineered Fc variant in complex with FcgRIIIa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
Synchrotron site | Photon Factory |
Beamline | AR-NE3A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2012-02-17 |
Detector | ADSC QUANTUM 270 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 75.030, 72.490, 163.480 |
Unit cell angles | 90.00, 91.15, 90.00 |
Refinement procedure
Resolution | 25.000 - 2.780 |
R-factor | 0.238 |
Rwork | 0.236 |
R-free | 0.27400 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.947 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.030 | 2.850 |
High resolution limit [Å] | 2.780 | 2.780 |
Rmerge | 0.079 | 0.619 |
Number of reflections | 43675 | |
<I/σ(I)> | 13.91 | 2.09 |
Completeness [%] | 98.0 | 98.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.25 | 293 | 14.5% PEG 3350, 0.1M Bis-Tris, 0.2M Sodium Iodide, Streak seeding, pH 6.25, VAPOR DIFFUSION, HANGING DROP, temperature 293K |