3WMM
Crystal structure of the LH1-RC complex from Thermochromatium tepidum in C2 form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-01-25 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 0.9 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 227.341, 148.238, 161.792 |
Unit cell angles | 90.00, 117.59, 90.00 |
Refinement procedure
Resolution | 47.991 - 3.008 |
R-factor | 0.3151 |
Rwork | 0.314 |
R-free | 0.33740 |
Structure solution method | MIR |
RMSD bond length | 0.029 |
RMSD bond angle | 2.056 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Number of reflections | 78312 | |
<I/σ(I)> | 16.2 | 2 |
Completeness [%] | 82.3 | 65.5 |
Redundancy | 3.6 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 293 | 20mM Tris-HCl (pH 7.4), 50mM CaCl2, 0.54% DPC, 2% glycerol, 14% PEG 3000, VAPOR DIFFUSION, SITTING DROP, temperature 293K |