3WMJ

Crystal structure of EIAV vaccine gp45

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	200
Detector technology	CCD
Collection date	2011-05-09
Detector	ENRAF-NONIUS
Wavelength(s)	0.9794
Spacegroup name	P 63
Unit cell lengths	46.947, 46.947, 101.605
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.022 - 1.998
R-factor	0.1886
Rwork	0.187
R-free	0.22470
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.867
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.022	2.020
High resolution limit [Å]	1.998	1.998
Number of reflections	8643
Completeness [%]	11.0	95

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	4.6	293	0.2M NaCl, 0.1M sodium acetate trihydrate, 30%(v/v) (+/-)-2-methyl-2,4-pentanediol, pH 4.6, EVAPORATION, temperature 293K