3WLV
Thermostable urate oxidase from Bacillus sp. TB-90
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-04 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 133.571, 144.635, 70.794 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.924 - 1.747 |
| R-factor | 0.1602 |
| Rwork | 0.159 |
| R-free | 0.19000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.279 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.780 |
| High resolution limit [Å] | 1.747 | 1.747 |
| Rmerge | 0.094 | 0.377 |
| Number of reflections | 138568 | |
| <I/σ(I)> | 56.8 | 9.4 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 13.6 | 13 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 8 | 293 | 15% PEG 8000, 0.1M Tris-HCl, 0.07M potassium sulfate, EVAPORATION, temperature 293K |
