3WLD
Crystal structure of monomeric GCaMP6m
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ DW |
| Temperature [K] | 95 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-08-28 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 120.880, 120.880, 97.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.059 - 2.700 |
| R-factor | 0.1722 |
| Rwork | 0.170 |
| R-free | 0.21210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3sg4 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.084 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.440 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.087 | 0.377 |
| Number of reflections | 20011 | |
| <I/σ(I)> | 12.7 | 3.3 |
| Completeness [%] | 98.1 | 95.3 |
| Redundancy | 5.3 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 0.1M HEPES, 18% w/v PEG 3350, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
