3WJL

Crystal structure of IIb selective Fc variant, Fc(V12), in complex with FcgRIIb

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	95
Detector technology	CCD
Collection date	2012-04-24
Detector	RAYONIX MX225HE
Wavelength(s)	1.00
Spacegroup name	C 2 2 21
Unit cell lengths	156.710, 260.200, 56.860
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 2.860
R-factor	0.2322
Rwork	0.230
R-free	0.26960
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.075
Data reduction software	XDS
Data scaling software	SCALA (3.3.20)
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	56.850	56.850	2.880
High resolution limit [Å]	2.810	12.570	2.810
Rmerge		0.034	0.886
Rmeas		0.037	0.951
Rpim		0.015	0.344
Total number of observations		2332	15552
Number of reflections	28725
<I/σ(I)>	15.6	34.7	2.5
Completeness [%]	99.5	98.1	99.5
Redundancy	7.2	6.2	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	19% PEG 3350, 0.1M Bis-Tris, 0.2M potassium phosphate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K