3WJJ
Crystal structure of IIb selective Fc variant, Fc(P238D), in complex with FcgRIIb
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2011-11-30 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9780 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.850, 76.010, 115.080 |
| Unit cell angles | 90.00, 100.70, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.600 |
| R-factor | 0.2323 |
| Rwork | 0.230 |
| R-free | 0.27160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.980 |
| Data reduction software | XDS |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.500 | 2.670 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.062 | 0.367 |
| Number of reflections | 25455 | |
| <I/σ(I)> | 15 | 3.4 |
| Completeness [%] | 99.6 | 98.3 |
| Redundancy | 3.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 17% PEG 3350, 0.1M Bis-Tris, 0.2M ammonium acetate, 2.7% D-galactose, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
