3WIR
Crystal structure of kojibiose phosphorylase complexed with glucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 71.581, 104.464, 124.164 |
| Unit cell angles | 68.83, 86.02, 90.06 |
Refinement procedure
| Resolution | 46.260 - 2.050 |
| R-factor | 0.19374 |
| Rwork | 0.191 |
| R-free | 0.25055 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h54 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.853 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.120 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Number of reflections | 203954 | |
| <I/σ(I)> | 14.5 | 2.3 |
| Completeness [%] | 97.3 | 96.2 |
| Redundancy | 3.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 5mM D-glucose, 5mM sodium phosphate, 10%(v/v) 2-propanol, 10%(w/v) PEG3350, 0.1M Tris-HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
