3WIC
Structure of a substrate/cofactor-unbound glucose dehydrogenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-09 |
| Detector | RIGAKU SATURN A200 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 79.088, 122.175, 87.428 |
| Unit cell angles | 90.00, 109.92, 90.00 |
Refinement procedure
| Resolution | 49.080 - 2.600 |
| R-factor | 0.2075 |
| Rwork | 0.205 |
| R-free | 0.25650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cd9 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.598 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 48068 | |
| Completeness [%] | 99.9 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | PEG 400, 1,2-propanediol, HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
