3WH2
Human Mincle in complex with citrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-01-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 31 |
| Unit cell lengths | 49.213, 49.213, 43.550 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.391 - 1.300 |
| R-factor | 0.1294 |
| Rwork | 0.128 |
| R-free | 0.15600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fm5 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.118 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.391 | 1.346 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.078 | 0.518 |
| Number of reflections | 29014 | |
| <I/σ(I)> | 23 | 3.1 |
| Completeness [%] | 100.0 | 99.31 |
| Redundancy | 5.5 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 293 | 1.0M lithium chloride, 0.1M citric acid, 20%(w/v) PEG3350, pH 4.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
