3WGX

Crystal structure of ERp46 Trx2 in a complex with Prx4 C-term

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2013-04-17
Detector	RAYONIX MX225HE
Wavelength(s)	0.9
Spacegroup name	P 1
Unit cell lengths	35.098, 36.406, 40.594
Unit cell angles	81.17, 87.47, 85.40

Refinement procedure

Resolution	26.785 - 0.920
R-factor	0.1405
Rwork	0.140
R-free	0.15800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.500
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: dev_1386))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.785	0.940
High resolution limit [Å]	0.920	0.920
Number of reflections	126561
Completeness [%]	89.9	62.3
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.8	293	0.1 M MES, 20% PEG3350 , pH 6.8, VAPOR DIFFUSION, SITTING DROP, temperature 293K