3WGM
STAPHYLOCOCCUS AUREUS FTSZ T7 mutant substituted for GAN bound with GTP, DeltaT7GAN-GTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-10 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.530, 89.640, 66.580 |
| Unit cell angles | 90.00, 96.11, 90.00 |
Refinement procedure
| Resolution | 44.820 - 2.091 |
| R-factor | 0.1947 |
| Rwork | 0.193 |
| R-free | 0.22670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3voa |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.023 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.220 | |
| High resolution limit [Å] | 2.090 | 6.220 | 2.090 |
| Rmerge | 0.118 | 0.054 | 0.778 |
| Rmeas | 0.063 | 0.905 | |
| Number of reflections | 30298 | 1193 | 4729 |
| <I/σ(I)> | 8.13 | 21.11 | 1.98 |
| Completeness [%] | 98.4 | 98.8 | 95.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M Sodium HEPES, 27.5%(w/v) PEG2000MME, vapor diffusion, sitting drop, temperature 293K |
