3WFW
Crystal Structure of the Closed Form of the HGbRL's Globin Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-08-23 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 67.240, 54.400, 44.710 |
| Unit cell angles | 90.00, 106.17, 90.00 |
Refinement procedure
| Resolution | 27.514 - 1.650 |
| R-factor | 0.1519 |
| Rwork | 0.151 |
| R-free | 0.16890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.201 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.8.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.690 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.025 | 0.061 |
| Number of reflections | 38021 | |
| <I/σ(I)> | 22.01 | 10.96 |
| Completeness [%] | 89.6 | 87.3 |
| Redundancy | 2.3 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 50% 2-methyl-2,4-pentanediol, 0.1M Tris-HCl, 0.2M (NH4)2HPO4, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
