3WFV
HIV-1 CRF07 gp41
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-09-16 |
| Detector | ENRAF-NONIUS |
| Wavelength(s) | 0.9793 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 46.920, 46.920, 185.051 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.102 - 1.800 |
| R-factor | 0.2219 |
| Rwork | 0.220 |
| R-free | 0.25820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.226 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.102 | |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.125 | |
| Number of reflections | 7326 | |
| <I/σ(I)> | 17.5 | |
| Completeness [%] | 95.8 | 95.76 |
| Redundancy | 11.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 8% PEG6000, 0.1M Tris, 40% MPD, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
