3WEV
Crystal structure of the Schiff base intermediate of L-Lys epsilon-oxidase from Marinomonas mediterranea with L-Lys
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-05-30 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 194.992, 126.164, 70.865 |
| Unit cell angles | 90.00, 99.03, 90.00 |
Refinement procedure
| Resolution | 46.860 - 1.980 |
| R-factor | 0.15187 |
| Rwork | 0.150 |
| R-free | 0.18914 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3weu |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.838 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.020 |
| High resolution limit [Å] | 1.980 | 1.990 |
| Rmerge | 0.097 | 0.362 |
| Number of reflections | 115096 | |
| <I/σ(I)> | 29.1 | 7 |
| Completeness [%] | 99.2 | 97.2 |
| Redundancy | 5.8 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 1.6M ammonium sulfate, 10%(v/v) 1,4-dioxane, 0.1M MES/NaOH, 75mM L-Lys, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
