3WEU
Crystal structure of the L-Lys epsilon-oxidase from Marinomonas mediterranea
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-20 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.1000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 194.089, 125.746, 70.591 |
| Unit cell angles | 90.00, 99.22, 90.00 |
Refinement procedure
| Resolution | 47.540 - 1.930 |
| R-factor | 0.14337 |
| Rwork | 0.143 |
| R-free | 0.17833 |
| Structure solution method | SAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.946 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.930 | 1.940 |
| Rmerge | 0.065 | 0.240 |
| Number of reflections | 124499 | |
| <I/σ(I)> | 13.1 | 6.8 |
| Completeness [%] | 99.4 | 92.5 |
| Redundancy | 13.1 | 9.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 1.6M ammonium sulfate, 10%(v/v) 1,4-dioxane, 0.1M MES/NaOH, 0.2M NaI, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
