3WEE

Structure of the full-length yeast Arp7-Arp9 Heterodimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-09-02
Detector	PSI PILATUS 6M
Wavelength(s)	1.00608
Spacegroup name	P 31 2 1
Unit cell lengths	103.774, 103.774, 184.451
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.730 - 3.100
R-factor	0.2012
Rwork	0.199
R-free	0.21990
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.112
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX (1.8.2_1309)
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.732	3.270
High resolution limit [Å]	3.100	3.100
Rmerge	0.103	0.493
Number of reflections	21509
<I/σ(I)>	18.1	4.4
Completeness [%]	99.9	99.9
Redundancy	9	9.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	10.5	277	2M sodium/potassium phosphate, 100mM CAPS/NaOH, pH 10.5, 200mM lithium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 277K