3WE9
The crystal structure of YisP from Bacillus subtilis subsp. subtilis strain 168
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13C1 |
| Synchrotron site | NSRRC |
| Beamline | BL13C1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-26 |
| Detector | MX300HE |
| Wavelength(s) | 0.97622 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.966, 77.576, 91.378 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.920 |
| R-factor | 0.211 |
| Rwork | 0.211 |
| R-free | 0.23300 |
| Structure solution method | MIR |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.990 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.069 | 0.482 |
| Number of reflections | 24603 | |
| <I/σ(I)> | 32.7 | 5.5 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.8 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 298 | 1 M LiCl, 0.1 M Bicine pH 9.0, 18-20%(w/v) polyethylene glycol 6000, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
