3WCZ
Crystal structure of Bombyx mori aldo-keto reductase (AKR2E4) in complex with NADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 325 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.089, 55.359, 59.197 |
| Unit cell angles | 90.00, 106.80, 90.00 |
Refinement procedure
| Resolution | 34.500 - 1.300 |
| R-factor | 0.152 |
| Rwork | 0.151 |
| R-free | 0.16400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hdj |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.502 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.503 | |
| High resolution limit [Å] | 1.300 | 1.300 |
| Number of reflections | 69041 | |
| Completeness [%] | 98.5 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295.15 | 1M SODIUM CITRATE, 0.1M CACODYLATE (PH 6.5), VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 295.15K |
