3WCY
Murine Ifnar1 in complex with interferon-beta
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9436 |
| Spacegroup name | P 43 |
| Unit cell lengths | 54.591, 54.591, 239.801 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 17.990 - 2.900 |
| R-factor | 0.2392 |
| Rwork | 0.237 |
| R-free | 0.28560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3se4 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.040 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.064 | 0.550 |
| Number of reflections | 15228 | |
| <I/σ(I)> | 11.7 | 2.4 |
| Completeness [%] | 98.7 | 100 |
| Redundancy | 4 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 297 | 12% PEG 3350, 8% tacsimate pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
