3WCK
Crystal structure of monomeric photosensitizing fluorescent protein, Supernova
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-10 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 1 |
| Unit cell lengths | 60.252, 89.304, 109.885 |
| Unit cell angles | 70.57, 89.95, 71.62 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.184 |
| Rwork | 0.181 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3a8s |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.942 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.070 | 0.323 |
| Number of reflections | 88149 | |
| <I/σ(I)> | 23.5 | 5.1 |
| Completeness [%] | 97.9 | 97.1 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 293 | 0.1M SODIUM ACETATE, 2.2M SODIUM CHLORIDE, 0.2M LITHIUM SULFATE, pH 4.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
