3WBJ
Crystal structure analysis of eukaryotic translation initiation factor 5B structure II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-06 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 120.690, 120.690, 168.260 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.393 - 2.495 |
| R-factor | 0.2175 |
| Rwork | 0.215 |
| R-free | 0.25580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3wbi |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.938 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 44.393 | 2.650 | |
| High resolution limit [Å] | 2.490 | 7.390 | 2.490 |
| Rmerge | 0.080 | 0.029 | 0.520 |
| Number of reflections | 47589 | ||
| <I/σ(I)> | 35.07 | 94.16 | 7.26 |
| Completeness [%] | 99.6 | 99 | 98 |
| Redundancy | 23.33 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 293 | 0.1M Tris-HCl pH 8.0, 40% PEG 400, 0.2M Li2SO4, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
