3WBD
Crystal structure of anti-polysialic acid antibody single chain Fv fragment (mAb735) complexed with octasialic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2012-11-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 64 |
| Unit cell lengths | 161.174, 161.174, 45.884 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 100.000 - 1.800 |
| R-factor | 0.23446 |
| Rwork | 0.233 |
| R-free | 0.25883 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1plg |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.251 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 63488 | |
| <I/σ(I)> | 40.1 | 6.6 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 11.2 | 11.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 1.8M Ammonium citrate tribasic, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
