3WBB
Crystal Structures of meso-diaminopimelate dehydrogenase from Symbiobacterium thermophilum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-11-06 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5419 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 136.820, 99.950, 84.830 |
| Unit cell angles | 90.00, 112.25, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.930 |
| Rwork | 0.179 |
| R-free | 0.21650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bio |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.960 |
| High resolution limit [Å] | 1.930 | 5.240 | 1.930 |
| Rmerge | 0.103 | 0.081 | 0.263 |
| Number of reflections | 78773 | ||
| <I/σ(I)> | 28.9 | ||
| Completeness [%] | 99.5 | 99.3 | 97.1 |
| Redundancy | 13.2 | 14.1 | 11.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 298 | 1,4-dioxane, glycerol, NADP+, pH 6.2, vapor diffusion, sitting drop, temperature 298K |
