3WB9

Crystal Structures of meso-diaminopimelate dehydrogenase from Symbiobacterium thermophilum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2012-11-15
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5419
Spacegroup name	C 1 2 1
Unit cell lengths	126.249, 99.450, 80.822
Unit cell angles	90.00, 107.69, 90.00

Refinement procedure

Resolution	50.000 - 1.930
Rwork	0.171
R-free	0.20570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3bio
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.960
High resolution limit [Å]	1.930	5.240	1.930
Rmerge	0.058	0.047	0.198
Number of reflections	71273
<I/σ(I)>	21.4
Completeness [%]	99.7	100	97.5
Redundancy	8.6	8.7	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	MPD, Glycerol, NaCl, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K