3WAK
Crystal structure of the Archaeoglobus fulgidus oligosaccharyltransferase (O29867_ARCFU) in the apo form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL32XU |
Synchrotron site | SPring-8 |
Beamline | BL32XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-05-27 |
Detector | RAYONIX MX325HE |
Wavelength(s) | 1.0000 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 123.357, 123.357, 182.496 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.896 - 3.413 |
R-factor | 0.2213 |
Rwork | 0.219 |
R-free | 0.27200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3waj |
RMSD bond length | 0.003 |
RMSD bond angle | 0.787 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.900 | 3.460 |
High resolution limit [Å] | 3.400 | 3.410 |
Rmerge | 0.151 | |
Number of reflections | 19650 | |
<I/σ(I)> | 15.8 | 2.5 |
Completeness [%] | 99.5 | 100 |
Redundancy | 13.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.02M Tris-HCl, 22% PEG 550MME, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |