3WA3
Crystal structure of copper amine oxidase from arthrobacter globiformis in N2 condition
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-01-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 191.607, 63.036, 158.006 |
| Unit cell angles | 90.00, 117.44, 90.00 |
Refinement procedure
| Resolution | 29.932 - 1.550 |
| R-factor | 0.1826 |
| Rwork | 0.181 |
| R-free | 0.20990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iu7 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.581 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.610 |
| High resolution limit [Å] | 1.550 | 3.340 | 1.550 |
| Rmerge | 0.125 | 0.107 | 0.499 |
| Number of reflections | 232158 | ||
| <I/σ(I)> | 10.9 | ||
| Completeness [%] | 95.5 | 97.2 | 95.4 |
| Redundancy | 5 | 6.2 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 6.8 | 289 | 1.05 M potassium tartrate, 25 mM HEPES, pH 6.8, MICRODIALYSIS, temperature 289K |
