3WA2
High resolution crystal structure of copper amine oxidase from arthrobacter globiformis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-19 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.9 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 157.745, 62.379, 92.076 |
| Unit cell angles | 90.00, 112.10, 90.00 |
Refinement procedure
| Resolution | 31.970 - 1.080 |
| R-factor | 0.131 |
| Rwork | 0.130 |
| R-free | 0.15000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iu7 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.582 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 100.000 | 100.000 | 1.100 |
| High resolution limit [Å] | 1.080 | 2.930 | 1.080 |
| Rmerge | 0.062 | 0.052 | 0.444 |
| Number of reflections | 663537 | ||
| <I/σ(I)> | 20.6 | ||
| Completeness [%] | 95.4 | 93.7 | 63.3 |
| Redundancy | 4.1 | 5.9 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.8 | 289 | 1.05 M potassium tartrate, 25 mM HEPES (pH 6.8), MICRODIALYSIS, temperature 289K |
