3W9V
Crystal structure of refolded DING protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 94.340, 86.890, 88.640 |
| Unit cell angles | 90.00, 90.81, 90.00 |
Refinement procedure
| Resolution | 29.544 - 1.031 |
| R-factor | 0.174 |
| Rwork | 0.173 |
| R-free | 0.18400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v3q |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.041 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 44.320 | 1.060 | |
| High resolution limit [Å] | 1.030 | 4.610 | 1.030 |
| Rmerge | 0.043 | 0.017 | 0.588 |
| Rmeas | 0.020 | 0.717 | |
| Number of reflections | 346764 | 3957 | 24541 |
| <I/σ(I)> | 14.3 | 58.22 | 2.08 |
| Completeness [%] | 99.1 | 98.3 | 94.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | sitting drop | 298 | PEG 8000, NaCl, Tris, pH 7.0-9.0, sitting drop, temperature 298K |
