3W9R
Crystal structure of the high-affinity abscisic acid receptor PYL9/RCAR9 bound to ABA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 111.405, 111.405, 40.478 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| R-factor | 0.18893 |
| Rwork | 0.188 |
| R-free | 0.20447 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cnw |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.265 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.050 | 0.570 |
| Number of reflections | 23009 | |
| <I/σ(I)> | 60.7 | 4.73 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 10.7 | 10.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 293 | 16.5 (w/v)% PEG 1500, maric acid-MES-Tris (MMT) buffer, pH 7.0, VAPOR DIFFUSION, temperature 293K |
