3W9P
Crystal structure of monomeric FraC (second crystal form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.980, 60.510, 80.840 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.270 - 2.100 |
| R-factor | 0.1646 |
| Rwork | 0.162 |
| R-free | 0.21560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lim |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.441 |
| Data reduction software | MOSFLM |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.5.1) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.270 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.104 | 0.366 |
| Number of reflections | 17493 | |
| <I/σ(I)> | 13.5 | 4.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.4 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 100mM sodium citrate, 20% isopropanol, 16% PEG 4000, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
