3W9G
Crystal structure of the ankyrin repeat domain of chicken TRPV4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2012-06-08 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 104.952, 48.267, 134.029 |
| Unit cell angles | 90.00, 101.79, 90.00 |
Refinement procedure
| Resolution | 37.569 - 2.000 |
| R-factor | 0.1798 |
| Rwork | 0.179 |
| R-free | 0.21150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3jxi |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.119 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.103 | 0.644 |
| Number of reflections | 89687 | |
| <I/σ(I)> | 17.6 | 2.5 |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 5.6 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 110-120mM sodium MES-HCl buffer pH 6.5, 1-2% PEG4000, 11-12% MPD, 55-60mM KH2PO4, vapor diffusion, sitting drop, temperature 293K |
