3W6V

Crystal structure of the DNA-binding domain of AdpA, the global transcriptional factor, in complex with a target DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NE3A
Synchrotron site	Photon Factory
Beamline	AR-NE3A
Temperature [K]	95
Detector technology	CCD
Collection date	2010-03-07
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0000
Spacegroup name	C 2 2 21
Unit cell lengths	76.510, 100.640, 100.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.500 - 2.950
R-factor	0.23787
Rwork	0.236
R-free	0.27830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1d5y 1bl0
RMSD bond length	0.009
RMSD bond angle	0.881
Data reduction software	HKL-2000
Data scaling software	XDS
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.870
High resolution limit [Å]	2.800	2.800
Rmerge	0.077	0.077
Number of reflections	9885
<I/σ(I)>	11.93	11.93
Completeness [%]	98.4	98.4
Redundancy	5.4	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.1	277	0.1M sodium HEPES, 10%(v/v) 2-propanol, 16%(w/v) PEG4000, pH 7.1, VAPOR DIFFUSION, SITTING DROP, temperature 277K