3W6U

Crystal structure of NADP bound L-serine 3-dehydrogenase from Hyperthermophilic Archaeon Pyrobaculum calidifontis

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-30
Detector	Pilatus 2M-F
Wavelength(s)	0.9789, 0.9792, 0.9640
Spacegroup name	C 1 2 1
Unit cell lengths	121.603, 56.827, 56.571
Unit cell angles	90.00, 106.69, 90.00

Refinement procedure

Resolution	39.750 - 2.000
Rwork	0.227
R-free	0.24200
Structure solution method	MAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.000
Number of reflections	25316

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	293	30% PEG2000, 0.2 M ammonium sulfate, 0.1M acetate buffer , pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K