3W5X
MamM-CTD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Collection date | 2011-11-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.939 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 36.882, 94.559, 53.542 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.280 - 1.600 |
| R-factor | 0.1714 |
| Rwork | 0.170 |
| R-free | 0.20650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3byp |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.149 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 4.340 | 1.600 |
| Rmerge | 0.058 | 0.039 | 0.480 |
| Number of reflections | 12559 | ||
| <I/σ(I)> | 18.4 | ||
| Completeness [%] | 98.2 | 85.8 | 83.4 |
| Redundancy | 6.1 | 5.1 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 25% PEG 4000, 0.2M Ammonium sulfate, 0.1M Sodium acetate , pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
