3W59

Crystal structure of Galectin-1 in the lactose-unbound state(P212121)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-11-29
Detector	RIGAKU JUPITER 210
Wavelength(s)	0.9790
Spacegroup name	P 21 21 21
Unit cell lengths	58.140, 111.557, 117.192
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.190 - 2.100
R-factor	0.212
Rwork	0.212
R-free	0.23100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gzw
RMSD bond length	0.008
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS (1.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.190	2.140
High resolution limit [Å]	2.100	2.100
Rmerge		0.142
Number of reflections	45407
<I/σ(I)>		11.1
Completeness [%]	100.0	100
Redundancy		14.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.1	277	2.0M ammonium sulfate, 1% 2-Mercaptoethanol, pH 5.1, VAPOR DIFFUSION, HANGING DROP, temperature 277K