3W4R
Crystal structure of an insect chitinase from the Asian corn borer, Ostrinia furnacalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 65 |
| Unit cell lengths | 93.761, 93.761, 121.964 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.759 - 1.700 |
| R-factor | 0.1471 |
| Rwork | 0.147 |
| R-free | 0.15940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fxy |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.788 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.093 | 0.364 |
| Number of reflections | 66546 | |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 22.3 | 22.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 50mM HEPES, 100mM NaCl, 200mM ammonium sulfate, 20% PEG 3350, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
