3W42
Crystal structure of RsbX in complex with manganese in space group P1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-18 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 33.198, 41.649, 68.755 |
| Unit cell angles | 81.17, 89.96, 71.46 |
Refinement procedure
| Resolution | 20.000 - 1.060 |
| R-factor | 0.12847 |
| Rwork | 0.127 |
| R-free | 0.15492 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3w45 |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.216 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.100 |
| High resolution limit [Å] | 1.060 | 1.060 |
| Number of reflections | 155995 | |
| <I/σ(I)> | 23.6 | 2.3 |
| Completeness [%] | 90.3 | 71.5 |
| Redundancy | 3.6 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 298 | 20 % PEG 1000, 0.1M Tris-HCl, 5mM MgCl2, 3mM MnCl2, pH 8.5, VAPOR DIFFUSION, temperature 298K |
