3W3Y
Crystal structure of Kap121p bound to Nup53p
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-10-11 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 78.330, 131.440, 131.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.330 - 2.800 |
R-factor | 0.25309 |
Rwork | 0.251 |
R-free | 0.29672 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3w3t |
RMSD bond length | 0.011 |
RMSD bond angle | 1.576 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.990 | 2.940 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 32341 | |
Completeness [%] | 95.2 | 79.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1M HEPES, 10% 2-propanol, 24% PEG 20000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |