3W3W

Crystal structure of Kap121p bound to Ste12p

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2012-03-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	77.570, 126.160, 130.780
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.180 - 2.200
R-factor	0.21733
Rwork	0.215
R-free	0.25622
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3w3t
RMSD bond length	0.016
RMSD bond angle	1.864
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.210	2.250
High resolution limit [Å]	2.200	2.200
Number of reflections	62953
Completeness [%]	95.9	75.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	0.1M HEPES, 10% 2-propanol, 24% PEG 20000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K