3W3G
Crystal structure of human TLR8 (unliganded form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Detector technology | CCD |
| Collection date | 2012-10-25 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 137.525, 101.970, 141.340 |
| Unit cell angles | 90.00, 104.75, 90.00 |
Refinement procedure
| Resolution | 34.380 - 2.300 |
| R-factor | 0.18461 |
| Rwork | 0.182 |
| R-free | 0.23622 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.611 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.300 |
| Number of reflections | 83700 |
| <I/σ(I)> | 9.6 |
| Completeness [%] | 99.8 |
| Redundancy | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 293 | 11.75% PEG8000, 0.2M NaCl, 0.1M Tris, pH 8.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
