3W38
Sugar beet alpha-glucosidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-01-30 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 83.534, 95.463, 107.651 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.670 - 2.790 |
| R-factor | 0.2293 |
| Rwork | 0.228 |
| R-free | 0.25880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.765 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 43.670 | 2.960 | |
| High resolution limit [Å] | 2.790 | 8.240 | 2.790 |
| Rmerge | 0.133 | 0.047 | 0.581 |
| Rmeas | 0.052 | 0.645 | |
| Number of reflections | 21842 | 943 | 3360 |
| <I/σ(I)> | 12.13 | 26.82 | 3.64 |
| Completeness [%] | 99.1 | 98.6 | 96.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 100mM ammonium sulfate, 50mM sodium acetate buffer, 18% PEG monomethylether 2000, pH 4.5, vapor diffusion, hanging drop, temperature 298K |
