3W1I

Crystal structure of the N-terminal truncated selenocysteine synthase SelA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	90
Detector technology	CCD
Collection date	2008-03-04
Detector	ADSC QUANTUM 270
Wavelength(s)	1.07117
Spacegroup name	P 1 21 1
Unit cell lengths	92.825, 280.936, 97.645
Unit cell angles	90.00, 113.45, 90.00

Refinement procedure

Resolution	48.941 - 3.192
R-factor	0.1769
Rwork	0.174
R-free	0.23540
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.220
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	DM
Refinement software	PHENIX (1.7.1_743)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.310
High resolution limit [Å]	3.192	3.200
Rmerge	0.090	0.674
Number of reflections	75145
<I/σ(I)>	22.4	2.5
Completeness [%]	99.5	96
Redundancy	7.4	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	120mM Tris-HCl, 11% PEG 4000, 400mM CaCl2, 160mM KCl, 5.0mM NiCl2, 50mM Na2S2O3, 50mM L-serine, 0.2M NaCl, 10mM 2-mercaptoethanol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
2