3W1G
Crystal Structure of Human DNA ligase IV-Artemis Complex (Native)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-10-10 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9795 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 68.260, 105.480, 121.080 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 59.462 - 2.550 |
R-factor | 0.1827 |
Rwork | 0.180 |
R-free | 0.23360 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3w1b |
RMSD bond length | 0.008 |
RMSD bond angle | 1.153 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 68.260 | 2.690 |
High resolution limit [Å] | 2.550 | 2.550 |
Rmerge | 0.085 | 0.562 |
Number of reflections | 27784 | |
<I/σ(I)> | 8.8 | 2 |
Completeness [%] | 96.0 | 97.5 |
Redundancy | 2.8 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | 2M ammonium sulfate, 10mM YCl, 100mM MES, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |