3W10
Aurora kinase A complexed to pyrazole aminoquinoline I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2010-10-01 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.488 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 89.950, 89.950, 164.302 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.540 - 2.700 |
| R-factor | 0.24906 |
| Rwork | 0.246 |
| R-free | 0.30897 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.712 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.540 | 3.000 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 10633 | |
| Completeness [%] | 70.0 | 70 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | PEG 3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
