3W0F

Crystal structure of mouse Endonuclease VIII-LIKE 3 (mNEIL3)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2010-07-07
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	115.580, 43.823, 77.645
Unit cell angles	90.00, 128.96, 90.00

Refinement procedure

Resolution	38.760 - 2.000
R-factor	0.197
Rwork	0.197
R-free	0.23500
Structure solution method	MIR
RMSD bond length	0.005
RMSD bond angle	1.100
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.090
High resolution limit [Å]	2.000	2.000
Rmerge	0.125	0.483
Number of reflections	37958
<I/σ(I)>	16.46	2.64
Completeness [%]	94.8	97.1
Redundancy	9.8	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277	18% PEG 3350, 0.15M DL-malic acid, 0.1M HEPES-NaOH, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K
1	VAPOR DIFFUSION, HANGING DROP	7	277	18% PEG 3350, 0.15M DL-malic acid, 0.1M HEPES-NaOH, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K