3VZH
Crystal Structure of CRISPR-associated Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6B |
| Synchrotron site | PAL/PLS |
| Beamline | 6B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-05-14 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 123.323, 43.781, 37.006 |
| Unit cell angles | 90.00, 90.75, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.700 |
| R-factor | 0.18522 |
| Rwork | 0.183 |
| R-free | 0.22613 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kg4 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.942 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 20990 | |
| Completeness [%] | 96.5 | 95.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 20% PEG3350, 0.2M sodium bromide, 0.1M Bis-Tris propane, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
